Regarding the 2 2,200 Americans on waiting lists to get lifesaving HIV/AIDS medications through the country’s AIDS Drug Assistance Program : In an effort to address the crisis, Senators Burr, Coburn and Enzi introduced S. 3401, the ACCESS ADAP Work, a bill which provides $126 million in unobligated stimulus funds to eliminate waiting lists through Fiscal Year 2010. The bill failed to gain the support of the Administration or Congress. The $126 million was a number provided by state AIDS directors as the quantity of additional federal government funding required to meet current plan needs given the number of people on waiting around lists, expected number of fresh budget and patients cuts per state.The usage of bacterial isolates produced from discarded stool samples and that do not have individual affected person identifiers is certainly exempt from regulations regarding research on human subjects. Existing scientific isolates from the 1991 outbreak in Peru, strain C6706 ; the 2008 outbreak in Bangladesh, strain MDC126 ; and the 1971 outbreak in Bangladesh, stress N16961 had been cultured as described in the Supplementary Appendix, obtainable with the full text of this article at NEJM.org. DNA Preparation and Sequencing We isolated genomic DNA from each of the C6, N5, M4, H1, and H2 strains and sequenced it using previously described methods.15 More specifically, we constructed DNA libraries comprising SMRTbell constructs, each which was bound to a DNA polymerase and sequenced in a manner similar to that described previously,16 using the PacBio RS sequencing system .